Summary of mechanism of formation and action of 5-HETE-PLs by human neutrophils. (A) Generation of the lipids is stimulated via receptor-dependent stimuli, including bacterial peptides, and intracellular signaling mediators. Hydrolysis of arachidonate by cPLA₂ is required, then oxidation by 5-LOX, reduction by GPX, and re-esterification into the phospholipid membrane. (B) HETE-PEs enhance superoxide generation and IL-8 release while inhibiting NET formation. fMLP indicates N-formyl-methionine-leucine-phenylalanine; PLC, phospholipase C; cPLA₂, cytosolic phospholipase A₂; FACL, fatty acyl CoA ligase; and GPX, glutathione peroxidase.