Effect of Unc119 G22V on CD4 T-cell proliferation. CD4 T cells were infected and sorted as in Figure 3A. Cells were then stimulated (stim.) with plastic-bound anti-CD3 and anti-CD28 Abs (3 of 28) or Concanavalin A (ConA) or left unstimulated (−) for 3 days. Cultures were pulsed with [³H] thymidine 6 hours before cell harvesting. [³H] thymidine incorporation was measured by liquid scintillation counter. N = 4 experiments using CD4 T cells from 4 different healthy donors, P < .05 (Mann-Whitney U test) was found for comparison of G22V and EV cells (*) and comparison of G22V and WT cells (#).