Generation of animals transplanted with RE;c-Kit^D814V– or RE;c-Kit^{T417IΔ418-419}–expressing cells. (A) Representation of MSCV-based retroviral constructs. LTR indicates long-terminal repeats; and IRES, internal ribosome entry site. (B) FACS analysis of NIH 3T3 cells transduced with MIV, c-Kit^Wt, c-Kit^D814V, or c-Kit^{T417IΔ418-419} retroviruses, demonstrating Vex expression (i), surface c-Kit (ii), and intracellular c-Kit (iii) levels. (C) Kaplan-Meier survival analysis of mice transplanted with BM cells expressing control Bex;Vex (n = 17), RUNX1-ETO (RE; n = 17), D814V (n = 15), T417IΔ418-419 (n = 11), RE;c-Kit^D814V (n = 20), or RE;c-Kit^{T417IΔ418-419} (n = 37) retroviruses. (D) Kaplan-Meier survival analysis depicting differing latencies of RE;c-Kit^D814V–associated neoplasia, which included AML (n = 9), MPN (n = 7), and pre-B-ALL phenotypes (n = 4).