GATA2 expression in normal BM and different morphologic, cytogenetic, and molecular AML subgroups. (A) Normalized expression levels of GATA2 in pretreatment samples of 237 children with de novo AML and 80 normal BM samples. Expression was significantly higher in AML compared with normal BM. Normalized expression levels of GATA2 in different morphological FAB subgroups (B), in cytogenetic subgroups (C), and in molecular subgroups including molecular aberrations (D) and AML with WT1 or EVI1 overexpression (E). GATA2 expression was determined using gene-expression arrays (Affymetrix HGU133 Plus 2.0 microarray, probe set 209710_at) or real-time quantitative PCR. Median values are depicted by the horizontal lines. The Mann-Whitney U test was used to compare expression levels between groups. The threshold for high versus normal GATA2 expression is based on median GATA2 in normal BM + 2 SD and is depicted as a dashed line.