SILAC-based phosphoproteomic analysis of CRLF2-dependent signaling. (A) Schematic illustration of our previous SILAC study (Zhong et al³³ ) which identified tyrosine phosphorylation sites induced by TSLP.³³  (B) The same SILAC approach was used to identify tyrosine phosphorylation sites regulated by CRLF2 in the presence of JAK2 R683G in Ba/F3 cells. IL3 was withdrawn for 6 hours and lysates were mixed, subjected to phosphotyrosine enrichment and the enriched phosphopeptides were analyzed by LC-MS/MS. (C) The heatmap shows differences between tyrosine phosphorylation regulated by TSLP and by CRLF2/JAK2 R683G.