Time course analysis of OVA intracellular trafficking. (A-B) CLSM examinations (central optical sections) of DCs that were allowed to take up OVA-FITC for 15 minutes and then were washed and chased with medium for the indicated intervals. Cells were analyzed using different markers to evaluate the localization of internalized OVA-FITC (green) into early endosomes (Rab5), late endosomes (Rab7), lysosomes (Lamp-1), and recycling vesicles (Rab11), all detected in red. To evaluate the recruitment of TAP-1 (red) and NOX-2 enzyme (red) to OVA⁺ organelles, cells also were counterstained with anti–TAP-1 and NOX-2 Abs. Colocalization areas were detected in yellow. Data are representative of 9 independent experiments.