Serum HS is highly elevated at the onset of GVHD. Lethally irradiated BALB/c recipients received either 1 × 107 B10.D2 TCD-BM only (allo-BM), 1 × 107 B10.D2 TCD-BM and 5 × 106 B10.D2 LCs (allo-BM + LC), or 1 × 107 BALB/c TCD-BM and 5 × 106 BALB/c LCs (Syn-BM + LC). (A) After transplantation, serum HS concentrations were determined by ELISA at the indicated time points; n = 2-5 samples per time point (*P < .05 comparing allo-BM + LC and allo-BM at the indicated time point. (B) To determine the half-maximal effective concentration of HS on DC stimulation, BALB/c DCs (2 × 105/well) were cultured for 24 hours with differing concentrations of HS in triplicate, and IL-6 production was tested by ELISA. Results are representative of 3 independent experiments.
