Platelet counts and anti-CD61 antibody levels in transferred mice. Induction of (A) thrombocytopenia and (B) anti-CD61 antibody production in irradiated SCID mice transferred with 1.5 × 104 splenocytes from naive BALB/c mice (N = 10, ○), CD61 KO mice immunized against BALB/c platelets (N = 15, □), or SCID mice transferred with 1.5 × 104 splenocytes from CD61 KO mice immunized against WT BALB/c platelets and treated biweekly with 2 g/kg IVIg (N = 15, ▵). (A) Thrombocytopenia at day 7 occurred in all irradiated mice as previously described.11 Data are platelet counts (×109/L; ± SEM) over time (days). The solid horizontal line represents the normal mean platelet count (± SEM; hatched lines) from 250 healthy SCID mice (mean platelet count = 720 ± 41 × 109/L, SD). (B) Anti-CD61 specific antibody production was performed by flow cytometry, and the data are expressed as percent gated BALB/c platelets. *P < .01, ITP mice (□) versus IVIg-treated mice (▵).
