Functional analysis of CML CD34⁺ cells following Bcr-Abl knockdown ± dasatinib. (A) Proliferation of GFP⁺ sorted cells treated ± 150nM dasatinib in physiologic growth factors over 12 days (n = 3). (B) Apoptosis measured in infected cells (gated on GFP) by annexin V and viaprobe (n = 2). (C) CFC (n = 4, P = .025) and (D) LTC-IC assays (n = 1). (E) Bcr-Abl levels were measured by Q-PCR on colonies from CFC assay (n = 7 colonies for sh-scrambled and 9 colonies for sh-Bcr-Abl). (F) The level of total p-CrkL was measured by Western blotting on pooled colonies from CFC assay (n = 1).