Figure 4
Disruption of the FANCD2 CUE domain does not impair DNA damage-inducible FANCD2 and FANCI posttranslational modification. (A) FA-D2 cells were untreated or treated with 250nM MMC. Twelve hours later pellets were collected and lysed and proteins resolved and immunoblotted with antibodies against FANCD2, FANCI, and tubulin. (B) FA-D2 cells were untreated or treated with 20 J/m2 UV-C irradiation. Six hours later pellets were collected, and WCLs were generated. For each sample, an aliquot of the WCLs was then treated with λ-phosphatase (λ-PP). Proteins were resolved and immunoblotted with antibodies against FANCD2, FANCI, and α-tubulin.
