Genome-wide DNA methylation analysis of sorted cells reveals widespread changes in MDS HSCs. (A) Hierarchal clustering and heatmap based on methylation profiling reveals differences between MDS HSCs and control HSCs. (B) Volcano plot based on difference of mean methylation and significance of the difference shows both aberrant hypermethylated and hypomethylated loci in MDS HSCs (Lin⁻CD34⁺CD38⁻). Red dots indicate P < .05 and fold change > 1 log2.