After pIpC-mediated NF-Ya deletion total BM cells of ko samples decrease in numbers, and HSC numbers remain unchanged. (A) BM cells were collected 96 hours after pIpC treatment; stained with lineage Abs, sca-1, c-kit, and CD48, CD150 (SLAM markers); and analyzed by FACS. The gating strategy to enumerate LSK, CD48⁺, CD150⁺ cells (E-MPPs) and LSK, CD48⁻, CD150⁺ (HSCs) is shown. Note, ko samples contain relatively more HSCs. Twenty-four (B), 60 (C), and 96 (D) hours after pIpC treatment BM cells were isolated from control and ko mice, and absolute cell numbers of different populations were enumerated as shown in panel A. Absolute cell numbers as indicated are shown (n = 5 in B and C; n = 3 in D); *P < .05 and **P < .01).