Inhibition of apoptosis does not influence Hsp70 subcellular localization.wtBcl2 was expressed by lentiviral transduction in 9 MDS and 2 control samples. (A) wtBcl2 inhibits apoptosis and delays erythroid cell differentiation. Flow cytometry for mitochondrial membrane depolarization as % HIDC^low cells and phosphatidylserine exposure as the percentage of annexin V⁺ cells in the GFP⁺ cell population (left panel) and glycophorin A expression as the percentage of positive cells (right panel). (B) Immunofluorescence for Hsp70 in 9 MDS and 2 control erythroblast samples at day 14 of the culture. Representative experiments (1 control and 3 MDS) are shown. Nucleus countercolored with DAPI (left panel). Quantification of Hsp70 nucleo/cytosolic ratio by using the ImageJ software.