Antibody deposition detected in target areas of lung and liver in diseased animals. Six-micrometer sections of frozen lung and liver tissues harvested at day 60 after transplantation were analyzed by immunofluorescence. Tissues were incubated with FITC-conjugated anti-mouse Ig. (A) Representative images for lung and liver from 3 individual experiments. n = 8. (B) Ig deposition was quantified on a 0-3 scale to determine the amount of antibody in the tissues. (C) Serum from animals given BM only and animals given BM plus splenocytes was collected at day 60 after transplantation and incubated with healthy B10.BR lung and liver tissue followed by FITC-conjugated anti-mouse Ig to detect the presence of tissue-specific antibodies in the serum of diseased animals. White arrows depict areas of Ig deposition. Fluorescence was detected with an Olympus FluoView 500 confocal laser scanning microscope at a magnification of 200×.