Animals receiving B cell–deficient BM show a decrease in pathology. B10.BR recipients were transplanted with WT BM or BM from μMT knockout mice with or without WT T cells. (A) At 60 days after transplantation, mice were anesthetized and artificially ventilated to measure pulmonary function parameters. (B) Collagen deposition was quantified from trichrome-stained samples as a ratio of blue area to total area of tissue. Quantification was performed with the analysis tool in Photoshop CS3. (C) Lung and liver tissues were harvested, and 6-μm frozen sections were stained with FITC-conjugated anti–mouse Ig for antibody deposition within the tissues. White arrows denote areas of Ig deposition. Images shown are representative images of 3 individual experiments; n = 4. *P < .05; ***P < .005; P = .4066 for lung BM (μMT) only vs BM (μMT) + T cells; P = .2860 for liver BM (μMT) only vs BM (μMT) + T cells.