IgG-opsonized HCMV stimulate IFNγ production by Vδ2^neg γδ T cells. (A) The long-term CD16^pos Vδ2^neg γδ T-cell line was incubated for 24 hours with HCMV alone, anti-HCMV specific IgGs alone, or IgG-opsonized HCMV. Blocking anti-CD16 (clone 3G8) or control mAbs were used in certain conditions. The indicated recombinant cytokines were added at the onset of culture. The amount of IFNγ released in the supernatant was quantified by ELISA. The experiment was performed in duplicate (mean ± SD) and is representative of 3 different experiments.