![Figure 2. L-leucine administration has no effect on mTOR but dampens the p53 activity in hematopoietic progenitors. (A) Flow cytometric quantification of phospho-Rps6 (Ser235/236) and phospho-4E-BP1 (Thr37/46) in myeloid progenitors (CD11b+c-Kit+), proerythroblasts (Ter119lowc-Kit+CD11b−), and mature erythroid precursors (Ter119+CD11b−c-Kit−) on day 14 (n = 6, 3, 5, and 7 for control, control + L-leucine, [D/D], and [D/D] + L-leucine, respectively). Mean fluorescence intensity relative to that of isotype control is presented. (B) p53 transcriptional targets were quantified using real-time PCR in preCFU-E/CFU-E and preGM/GMP populations sorted from individual mice on day 14 (n = 5, 13, and 13 for control, [D/D], and [D/D] + L-leucine, respectively). Error bars represent SD.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/120/11/10.1182_blood-2012-05-431437/4/zh89991295640002.jpeg?Expires=1735810686&Signature=aNbGaZ-BtPPtoRG7rZr8Nc~67QLvPO2bt-S9wooJ8ZRscLxBg~CAWeQhBaR-j4vPi87iuYOR0FpqxBbvXPW-4r2Nn-7TacwcIXAZJuyXkTmRnpT9LCiYm-puH9EQOvM69hj~~xdfIU0E6wS8kcl8kkFOuvc9wuAJzSgXiMQVEN~fW9WJlL5tcIlObpaO2P053RRNVZj1USw~B6LHnqeTUUjnOZNFJnf-ZCK0-q6MDgCGTAYIBs8fXvY8NO6uy8eeMQvejzMSiAJTp4yMUl3ETf1VG7QkG0fljMLtJOSKfUUp5cJkq5P6mCcW24SSoakOfCrO9deQ1baE0HX5GPdPKg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
L-leucine administration has no effect on mTOR but dampens the p53 activity in hematopoietic progenitors. (A) Flow cytometric quantification of phospho-Rps6 (Ser235/236) and phospho-4E-BP1 (Thr37/46) in myeloid progenitors (CD11b+c-Kit+), proerythroblasts (Ter119lowc-Kit+CD11b−), and mature erythroid precursors (Ter119+CD11b−c-Kit−) on day 14 (n = 6, 3, 5, and 7 for control, control + L-leucine, [D/D], and [D/D] + L-leucine, respectively). Mean fluorescence intensity relative to that of isotype control is presented. (B) p53 transcriptional targets were quantified using real-time PCR in preCFU-E/CFU-E and preGM/GMP populations sorted from individual mice on day 14 (n = 5, 13, and 13 for control, [D/D], and [D/D] + L-leucine, respectively). Error bars represent SD.
