Increased neutrophil recovery after heat treatment is dependent on IL-17. C57BL/6 or IL17ra^−/− mice were left untreated (NT), treated with heat treatment alone, TBI alone, or TBI followed 2 hours later with a 6-hour heat treatment. (A-B) Cell lysates from intestine of C57BL/6 mice that received either 3 Gy (A) or 6 Gy (B) TBI with or without heat treatment were collected, and ELISAs were performed using 50 μg lysate per well to determine concentrations of IL-17. (C) Ly6G⁺ cell counts were performed in IL17ra^−/− mice 7 and 14 days after TBI. (D) Serum from IL17ra^−/− mice that received 3 Gy TBI with or without heat treatment was collected, and ELISAs were performed to determine G-CSF concentrations. Each graph is a representative of at least 2 separate experiments. n = 5 mice per group. *P < .01, TBI alone group vs TBI followed by mild heating. n.d. indicates not detected.