Strong decrease in CD141 (BDCA-3)⁺ mDC counts and increase in CD16⁺ and particularly CD14⁺CD16⁺⁺ monocyte counts in the circulation of viremic patients compared with controls and virologically suppressed patients. (A) Dot plots of 11-color FACS analysis (described in supplemental Figure 1) of PBMCs from (top panels) a representative healthy donor and (bottom panels) a viremic patient. From left to right, BDCA-1⁺ mDCs (beige), BDCA-3⁺ mDCs (red), and BDCA-2⁺ pDCs (purple); CD14⁺⁺CD16⁻ classical monocytes (blue), CD14⁺⁺CD16⁺ intermediate monocytes (cyan), and CD11c⁺CD14⁺CD16⁺⁺ nonclassical monocytes (green and orange) are shown. The percentage of each cellular subset among CD45⁺ PBMCs is indicated in each dot plot. (B-C) Absolute counts in the blood from healthy donors (HIV⁻, cART⁻, n = 13), virologically suppressed patients (HIV⁺, cART⁺, n = 8), and viremic patients (HIV⁺, cART⁻, n = 15) of (B) mDC subsets and pDCs and (C) monocyte subsets are shown (P values were calculated using the Mann-Whitney test; bars indicate medians). (D) Ring graphical representation of the mean proportion of monocyte subsets among total monocytes in the healthy donors (HIV⁻; n = 13) and viremic patients (n = 15).