RanBP10−/− platelets show modestly altered adhesion under shear but spread normally. (A) Regular receptor density of platelet surface markers in RanBP10-null and control mice. (B) Platelet spreading on fibrinogen (top row) and collagen (bottom row) is unaffected in RanBP10-null platelets, showing the typical flat “fried-egg” appearance. (C) Phase contrast microscopy and quantitative analysis of surface coverage of wild-type and RanBP10−/− platelets aggregating on collagen under intermediate flow (1000 seconds−1 left panel) and high flow (7700 seconds−1 right panel) show overall unaltered adhesion. (D) Analysis of thrombus volume in integrative fluorescence intensities (IFI) and quantification shows that wild-type and RanBP10−/− platelets form equal-sized thrombi at each shear rates as depicted for 1000 seconds−1 left panel and 1700 seconds−1 right panel.
