Absence of LTB4/BLT1 axis enhances maturation of DCs and recruitment of TAA-phagocytosed DCs into TDLNs in KO/WGM mice in early phase. Two left axillary TDLNs were harvested from WT/W, KO/W, WT/WGM, or KO/WGM mice on day 2 (CD80 and CD86) or day 4 (CD40) after the tumor challenge (n = 3-5 per group). Data are representative dot plots (top panel) and the averages of mean fluorescence intensity (bottom panel) for CD11c⁺ cells expressing the following markers of (A) CD40, (B) CD80, or (C) CD86 in TDLNs harvested from 4 indicated experimental groups. Bar graphs represent mean ± SEM. *Significant difference (P < .05). (D) Migration of DCs that had phagocytosed TAAs to TDLNs in WT/WGM or KO/WGM mice on day 2 after the subcutaneous inoculation with PKH26-labeled WGM cells. Numbers in 2-dimensional dot plots reflect the positive ratio of PKH26⁺CD86⁺ cells relative to total CD11c⁺ cells. Representative data from at least 3 independent experiments with similar results are shown.