Absence of LTB4/BLT1 axis promotes systemic activation of TAA-specific Th1 and Th2 subsets in KO/WGM mice in intermediate phase. In vitro Th1/Th2 cytokine production profiles of splenocytes harvested from WT/W, KO/W, WT/WGM, or KO/WGM mice on day 10 after the FTC. Approximately 1 × 10⁶ splenocytes harvested were cultured with or without 4 × 10⁵ irradiated WEHI3B cells for 20 hours. The concentrations of mouse (A) IL-4, (B) IL-5, (C) IL-2, (D) IFN-γ, and (E) TNF-α in the culture supernatants (n = 3) were measured by cytometric bead array assay. Bar graphs represent mean ± SEM. *Significant differences (P < .05). Representative data from 3 independent experiments with similar results are shown.