SEMA6A contributes to the function of miR-27 in endothelial cells. HUVECs were transfected with SEMA6A siRNA (67nM), miR-27a/b inhibitor (10nM), and the respective controls as indicated. (A) Expression of SEMA6A or miR-27b was measured by quantitative RT-PCR after 24 hours; n = 4. (B) Spheroids were generated and sprouting was quantified; n = 4.