Inhibition of miR-27a/b increases the repulsive activity of endothelial cells. HUVECs were transduced with GFP lentivirus before transfection with the miR-27a/b inhibitor (10nM) or miR inhibitor-control (10nM). The following day, 1.5 × 103 transfected endothelial cells (green) were seeded on top of untreated endothelial cells (70% confluency). Cells were analyzed by video time lapse microscopy over 12.5 hours. (A) Representative images at time points 0 and 12 hours are shown. The cell-free areas surrounding single miR inhibitor/GFP-transfected endothelial cells are marked by a yellow line. (B) The cell-free areas surrounding single miR inhibitor/GFP-transfected endothelial cells were analyzed, and the ratio of the cell-free area at t = 12.5 hours and t = 0 hours is given as percentage of control. *P < .001. n = 32 cells and n = 3 experiments. (C) HUVECs were transfected with SEMA6A siRNA, miR-27a/b inhibitor, and the respective controls as indicated. Cells were tracked over 12.5 hours, and the ratio of the cell-free area is given as percentage of control. P < .01. n = 30 to 45 cells and n = 3 experiments.
