Phenotype of LZ and DZ B cells in mice and humans. (A) Cell-cycle (DNA content) profiles of human and mouse LZ and DZ cells (gated shown in supplemental Figure 1A). Multiple experiments (n = 5 for mouse and n = 3 for human) are quantified in the graphs on the right (error bar represents SD). (B) Forward scatter (FSC) and side scatter (SSC) profiles of human and mouse LZ, DZ, and naive B cells. Naive B cells are defined as CD19⁺IgD⁺CD83⁻ in human tonsil and as B220⁺FAS⁻CD38⁺ in mouse lymph node. Right panels: quantification of multiple experiments (n = 4 for mouse and n = 3 for human; bar represents SD). (C) Expression of selected markers by human and mouse LZ, DZ, and naive B cells. (D) Expression of selected B-cell markers by human LZ, DZ, and naive B cells. (E) Reproducibility of surface molecule expression between human samples. Graph represents quantification of multiple experiments (n = 2 for CD86, MHC class II; n = 3 for Igκ+λ; and n = 4 for the remaining markers; bar represents SD).