Combined effects of Ly49G2 and Ly49A NK-cell subset depletion on B10 or β2m−/− BMC rejection by B10.D2 recipients. Recipient mice received rIgG control, anti-NK1.1 (PK136), anti-Ly49G2 (4D11), and/or anti-Ly49A (YE1/32) for NK cell and NK-cell subset depletion 2 and one day before lethal irradiation. A total of 15 million of B10 (A) or 50 million of β2m−/− BMCs (B) were transfused to B10.D2 recipients. Seven days after transplantation, spleens were collected and CFU-c assay was performed (mean ± SEM). One-way ANOVA test was performed to determine whether the mean values were significantly different (P < .05). Each experiment was performed 2 or 3 times.