Metformin administration improves the function of FA bone marrow cells. (A) Representative pictures of spleens analyzed in the CFU-S assay. (B) Statistical analysis of CFU-S assays. Forty thousand donor bone marrow cells were injected intravenously into each recipient mouse. Data represent 3 or 4 donors in each group of mice, with 2 to 4 recipients for each donor. (C) Schematic chart to show the procedures of poly(I:C) experiments. Three-month-old mice were injected intraperitoneally with either poly(I:C) or saline at 8 mg/kg body weight. The mice were harvested either 2 weeks (for CFU-S assay) or 3 weeks (for CBC analysis) after the completion of poly(I:C) treatment. (D) Statistical analysis of CFU-S assays after poly(I:C) administration. Data represent 8 or 9 donors in each group of mice, with 2 to 4 recipients for each donor. Total recipients in each group ranged from 23 to 28 mice. (E) Statistical analysis of CBC tests after poly(I:C) administration. Data are pooled results from 11 to 17 mice each group for wild-type mice and 18 to 19 mice each group for Fancd2^−/− mice.