American Society of Hematology

Figure 3

$Figure 3. Meis1-deleted HSCs are deficient in maintenance of hematopoiesis. (A) Competitive repopulation assay was performed as described in the text. (B) Summary of peripheral blood CD45.2 chimerism (vertical axis) in recipient mice over time (horizontal axis) since transplantation. The groups are labeled to the right of the respective curve. Data are shown as means ± SEM (n = 5-8 per group). Also shown on the lower right is a representative PCR result on peripheral blood of recipient mice treated with vehicle (V) or tamoxifen (T). Bands labeled to the right represent, respectively, in numerical order floxed, wild-type, and deleted Meis1 alleles, showing that we achieved complete deletion of Meis1 by tamoxifen treatment. (C) Chimerism in the various lineages at 4 months. (D) Flow cytometry plots showing chimerism in the LT-HSC fraction of the BM of recipient mice at 4 months.$

Meis1-deleted HSCs are deficient in maintenance of hematopoiesis. (A) Competitive repopulation assay was performed as described in the text. (B) Summary of peripheral blood CD45.2 chimerism (vertical axis) in recipient mice over time (horizontal axis) since transplantation. The groups are labeled to the right of the respective curve. Data are shown as means ± SEM (n = 5-8 per group). Also shown on the lower right is a representative PCR result on peripheral blood of recipient mice treated with vehicle (V) or tamoxifen (T). Bands labeled to the right represent, respectively, in numerical order floxed, wild-type, and deleted Meis1 alleles, showing that we achieved complete deletion of Meis1 by tamoxifen treatment. (C) Chimerism in the various lineages at 4 months. (D) Flow cytometry plots showing chimerism in the LT-HSC fraction of the BM of recipient mice at 4 months.

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