Detection of intracellular HIV-1 p24 Ag production in immature MoDCs and primary autologous PHA-activated CD4 T lymphocytes. (A) Dot-plot representations of MoDCs (in pink), infected with primary HIV-1 or uninfected, and CD4 T lymphocytes (in green) in the coculture. We differentiated between MoDCs and CD4 T lymphocytes on flow cytometry by analyzing the expression of DC-SIGN⁺ (CD209⁺) and CD3⁺, respectively. The HIV-1 reverse transcriptase inhibitor AZT was added to the coculture at the same time as CD4 T lymphocytes as a negative control for HIV-1 replication. Experiments were performed in duplicate and the mean percentages of intracellular p24⁺ MoDCs or CD4 T lymphocytes are shown in the dot plot. (B) Curve for the correlation between the mean values of the percentage of infected MoDCs and infected primary PHA-activated CD4 T lymphocytes in coculture conditions. The Pearson correlation coefficient and its significance are shown. n = 38 experiments performed with cells from different healthy blood donors for panels A and B.