Induction of MoDC maturation by the anti–HIV-1 neutralizing mAb 4E10 in various culture conditions. The percentage of CD83⁺ DC-SIGN⁺ MoDCs was determined after 48 hours of culture in the presence or absence of virus, CD4 T lymphocytes, or Abs. Data are the means ± SD of at least 6 experiments performed with cells from different healthy blood donors. Groups were compared by 1-way ANOVA (Kruskal-Wallis test), and pairwise comparisons were based on t tests (Mann-Whitney test), with P < .05 considered significant.