PSTPIP2 deficiency promotes osteoclast development and MIP-1α production in vivo. (A) Toluidine blue staining (left panels) and quantitation (right panel) of the resorption pits on dentine implants removed at the indicated times after surgery. (B) Resorption pits (left panels) and quantitation (right panel) of dentine resorption at day 10 postimplant. (C) TRAP staining of dentine implants (left panels) and quantitation of the density (right panel) of TRAP⁺ cells on implants removed at day 5. (D) Serum levels of pro-osteoclastogenic factors in 6-month-old cmo (top panel) and 3-month-old Lupo (bottom panel) mice and WT controls at day 8 after surgery. (E) MIP-1α production by macrophages after a 24-hour incubation in medium with or without 120 ng/mL CSF-1. Data ± SEM; n indicates number of mice per group; and ns, not significantly different (P > .05).