PSTPIP2 deficiency enhances osteoclast development from purified precursors. (A) TRAP staining of c-Kit⁺ c-Fms^hi Mac-1^lo (LOCP) and c-Kit⁺ c-Fms^lo Mac-1^lo (EOCP) osteoclast precursor cultures at day 3 and day 6 of CSF-1 and RANKL-induced osteoclast differentiation in vitro. (B) Quantitation of TRAP⁺ mononuclear OCP (day 3) or OCLC cells (day 6) shown in panel A. Data ± SEM from 3 independent experiments. ns indicates not significant (P > .05). (C) Compared with WT controls, cultured Lupo EOCP form more TRAP-positive multinucleated osteoclasts.