GP1a treatment reduces both c-Fos induction and c-Jun phosphorylation. (A) DCs treated with PGE2 with or without GP1a for 40 minutes were fixed, permeabilized, stained intracellular with c-Fos antibody, and analyzed by FACS. (Top) Shaded area represents untreated cells. (Bottom) Black line represents GP1a + PGE2, gray line represents PGE2, and broken black line represents GP1a. (B) DCs were treated with PGE2 with or without GP1a for various time periods and analyzed for phospho-c-Jun by Western blot. Densitometric analyses are plotted in graphs normalizing phospho-c-Jun to total c-Jun. Data are representative of 2 (B) and 3 (A) independent experiments.