Cxcl4^−/− mice display a reduction in HSC compartment. (A) BM (left) and spleen (right) from WT mice (n = 6) or Cxcl4^−/− mice (n = 6) were harvested and total cellularity assessed. (B) Numbers of LSK cells for BM (left) and spleen (right) from WT mice or Cxcl4^−/− mice. (C) Numbers of HSC, MPP, HPC-1, and HPC-2 in the BM (left) and spleen (right) from WT mice or Cxcl4^−/− mice are shown. (D) Numbers for BM (left) and spleen (right) erythroid, granulocyte, and B-cell compartments from WT and Cxcl4^−/− mice are shown. (E) Numbers for T-cell populations in the WT and Cxcl4^−/− spleen identified with CD4 and CD8 markers are shown. (F) Numbers for T-lineage populations in the WT and Cxcl4^−/− thymi were identified with CD4 and CD8 markers. Statistical analysis was performed using an unpaired two-tailed Student t test. All error bars indicate SEM of the mean (*P < .05; ***P < .001).