Enhanced IRF7 and p65 phosphorylation in myrPKB-expressing pDCs. CD34⁺ HPCs were transduced and cultured as described in the legend for Figure 5. HSV was added during the last 5 hours of culture. Expression of eGFP, CD123, BDCA-2, phosphorylated IRF7 (p-IRF7), and phosphorylated p65 (p-p65) was analyzed by flow cytometry. The percentage of eGFP⁺CD123⁺BDCA-2⁺ pDCs expressing p-IRF7 (A) or p-p65 (B) was determined. Representative FACS plots showing eGFP⁺ pDCs and percentages per independent experiment are shown (n = 3).