HSPC frequencies in NHD13⁺ Tg and control mice. Bone marrow (BM) cells from wild-type and NHD13⁺ Tg mice were harvested at 4 months of age and prepared for flow cytometric analysis. (A) Representative FACS plots show LSK, CD34, Flt3, and CD150 expression within WT and NHD13⁺ Tg mice BM cells. (B) The frequencies of LSK cells, ST-HSCs (LSKCD34⁺Flt3⁻) and LMPPs (LSKCD34⁺Flt3⁺) in WT and NHD13⁺ Tg mouse BM (mean ± SD, n = 7, *P < .05, **P < .01). (C) The frequency and (D) absolute number of CD150⁺ LT-HSCs (CD150⁺LSKCD34⁻Flt3⁻) in WT and NHD13⁺ Tg mouse BM are shown (mean ± SD, n = 7, *P < .05). (E left panel) The FACS plots and gating strategies used to identify CMPs (Lin⁻Sca-1⁻ cKit⁺ IL-7Rα⁻CD34⁺FcgR^low), GMPs (Lin⁻Sca-1⁻cKit⁺IL-7Rα⁻CD34⁺FcgR^high), and MEPs (Lin⁻Sca-1⁻cKit⁺IL-7Rα⁻CD34⁻FcgR^low) within the Lin⁻IL-7Rα⁻cKit⁺ population. Right panel, The frequencies of CMPs, GMPs, and MEPs in WT and NHD13⁺ Tg mice BM (mean ± SD, n = 8, **P < .01). (F left panel) Representative FACS plots of the gating strategy to identify the CLPs (Flt3⁺Lin⁻IL-7Rα⁺ckit^intSca-1^int) population. (Right panel) The frequency of CLPs in WT and NHD13⁺ Tg mice BM (mean ± SD, n = 8, **P < .01).