Increased p53 level in NHD13⁺ bone marrow cells. The intracellular p53 level was measured by flow cytometry. (A) Representative FACS profiles of p53 protein levels in WT (gray) and NHD13⁺ Tg (black) mouse bone marrow (BM) LSK (Lin⁻Sca-1⁺cKit⁺) cells. The graph on the right indicates the mean percentage of p53⁺ cells present (n = 5, ***P < .001). (B) Representative FACS profiles of p53 protein levels in WT (gray) and NHD13⁺ Tg (black) mice BM CD71⁺Ter119⁻ cells. The bar graph on the right indicates the mean percentage of p53⁺ cells present (n = 5, *P < .05). (C) Representative FACS profiles of p53 protein levels in WT (gray) and NHD13⁺ Tg (black) mice BM CD71⁺Ter119⁺ cells. Data shown on the right are the mean percentage of p53⁺ cells present (n = 5, P = .052).