The loss of 1 or 2 p53 alleles does not rescue the MDS phenotype induced by NHD13 fusion protein. (A) Representative staining profiles for LSK cells, CD34, Flt3, and CD150 expression within BM cells of NHD13⁺, NHD13⁺p53^+/−, and NHD13⁺p53^−/− mice with MDS, compared with WT controls. (B) The frequencies of LSK cells, ST-HSCs, LMPPs, CD150⁺ LT-HSCs, and the total number of CD150⁺ LT-HSCs in NHD13⁺, NHD13⁺p53^+/−, and NHD13⁺p53^−/− mice with MDS are compared with WT control mice (mean ± SD, *P < .05, **P < .01). (C) Representative FACS profiles for CMPs (Lin⁻Sca-1⁻ cKit⁺ IL-7Rα⁻CD34⁺FcgR^low), GMPs (Lin⁻Sca-1⁻cKit⁺IL-7Rα⁻CD34⁺FcgR^high), and MEPs (Lin⁻Sca-1⁻cKit⁺IL-7Rα⁻CD34⁻FcgR^low) within the Lin⁻IL-7Rα⁻cKit⁺ population for the NHD13⁺, NHD13⁺p53^+/−, and NHD13⁺p53^−/− mice with MDS, compared with the WT control mice. (D) The frequencies of CMPs, GMPs, and MEPs in the NHD13⁺, NHD13⁺p53^+/−, and NHD13⁺p53^−/− mice with MDS, compared with WT control mice (mean ± SD, *P < .05, **P < .01). (E) Representative FACS profiles showing CD71 and Ter119 staining of BM cells isolated from NHD13⁺, NHD13⁺p53^+/−, and NHD13⁺p53^−/− mice with MDS, compared with WT control. (F) The frequencies of Ter119⁺ and CD71⁻Ter119⁺ cells in the NHD13⁺, NHD13⁺p53^+/−, and NHD13⁺p53^−/− mice with MDS, compared with WT controls (mean ± SD, *P < .05, **P < .01). The mice used for this figure were between 4 and 5 months old; n = 8 for each genotype.