Spreading of LAD-III NK cells on ICAM-1 is intact, whereas shear-resistant adhesion and migration are impaired. (A) The figure shows the LFA-1–dependent adhesion under shear force. NK cells derived either from a healthy control (□) or from the LAD-III patient (■) were settled for 1 minute on ICAM-1-Fc–coated surfaces (350 sites/μm²) and were then subjected to increasing shear forces. Figure shows 1 representative experiment of 3 performed. Data are mean ± SD. Error bars (SD) are derived from triplicates. *P < .05. (B) Frequency histograms of the speed of cell migration on ICAM-1–coated slides measured at 5-second intervals, from healthy control NK cells or from the LAD-III patient NK cells, which had either been stimulated with 5mM MgCl₂ or left untreated. The total number of time points analyzed for each experimental condition is indicated. (C) The average migration speeds of healthy control NK cells, LAD-III patient NK cells, or Mg²⁺ stimulated LAD-III patient NK cells on ICAM-1. Data are mean ± SEM, derived from 3 separate experiments. **P < .005. NS indicates not significant. (D) Bright-field and IRM images of NK cell surface attachment and spreading on ICAM-1 from a healthy control (left) or from the LAD-III patient (right). Scale bar represents 10 μm. (E) Quantification of the area of surface attachment for n > 60 cells. Data are mean ± SEM. **P < .005.