NK cell cytotoxicity is LFA-1–dependent, kindlin-3–independent. (A) Bulk NK cells derived from a healthy control or from the LAD-III patient were assayed for killing of the 1106mel melanoma cell line. The various NK cells were either left untreated or treated with anti-LFA-1 mAb (168.6), XVA, (a β2-integrin chemical blocker), or with a control mAb (12E7). The E:T ratio was 5:1. Figure shows 1 representative experiment of 6 performed. Data are mean ± SD. Error bars (SD) are derived from triplicates. *P < .05. NS indicates not significant. (B) NK cells derived from the LAD-III patient and from a healthy control were incubated for 2.5 hours with 1106mel cells at 37°C with and without the various reagents described in panel A. The expression of CD107a was analyzed by FACS on the CD56+ cells. The E:T ratio was 1:1. Data are mean ± SD. Error bars (SD) are derived from triplicates. *P < .05. NS indicates not significant. Figure shows 1 representative experiment of 2 performed. (C) Bulk NK cells derived from a healthy control or from the LAD-III patient were assayed for killing of various target cells (indicated above the graphs) at different E:T ratios (as indicated on the x-axis). The NK cells were treated with anti-LFA-1 mAb (168.6) or with a control mAb (12E7). Figure shows 1 representative experiment of 2 performed. Data are mean ± SD. Error bars (SD) are derived from triplicates. *P < .05. **P < .005. ***P < .0005.
