Reduced apoptosis and increased cell cycle entry of stem/progenitor cells in RUNX1(41-214) animals. (A) Homing assay of KSL cells from MigR1 (□; n = 3) and RUNX1(41-214) (■; n = 3); numbers represent KSLs homed to bone marrow per 1 million injected cells. (B) Annexin V staining of stem/progenitor cells in MigR1 (□; n = 3) and RUNX1(41-214) (■; n = 3) animals. (C) Representative cell cycle analysis with Pyronin Y/7-AAD in MigR1 and RUNX1(41-214) animals. (D) Cell cycle analysis of stem/progenitor cells of in MigR1 (□) and RUNX1(41-214) (■) animals that received a transplant. Results are average of 3 independent experiments. Three to 6 animals per group were used in each experiment. Only statistically significant P values are shown (*P < .05). All analyses were performed at 4-6 months after transplantation. c-Kit⁺ indicates EGFP⁺/Lin⁻/IL7Rα⁻/c-Kit⁺/Sca-1⁻; KSL, EGFP⁺/Lin⁻/IL7Rα⁻/c-Kit⁺/Sca-1⁺; PY, pyronin Y; KSL CD48⁺, EGFP⁺/Lin⁻/IL7Rα⁻/c-Kit⁺/Sca-1⁺/CD48⁺; and KSL CD48⁻CD150⁺, EGFP⁺/Lin⁻/IL7Rα⁻/c-Kit⁺/Sca-1⁺/CD48⁻/CD150⁺.