GPR34 overexpression enhances cell proliferation of HeLa and lymphoma B cells. (A) Proliferation of HeLa vector control, WT, or DRY cells alone or in the in the presence of 25 to 100μM PD98059 was analyzed. *Compared with the vector control, WT cells have a significant increase in cell proliferation (P < .0001). (B) Tumor colony-forming assays were performed on HeLa vector control, WT, and DRY cells. A media control with no cells was included as a negative control. Photomicrograph of tumor colony in inset, scale bar, 100μM. *Compared with the vector control (P = .05) or DRY (P = .01), WT cells have a significant increase in colony formation (n = 3). (C) Proliferation of OCI-Ly19 vector control or WT cells alone or in the presence of 50 to 100μM PD98059 was analyzed (n = 3, representative experiment shown). *Compared with the vector control, WT cells have a significant increase in cell proliferation (P = .0002).