Loss of KLF7 does not affect bone marrow repopulating activity. The frequency of KLS (A) and colony-forming cells (B) in the fetal liver from 13.5-14.5-dpc Klf7^−/− and Klf7^+/+ embryos is shown. Data represent 2 or 3 independent experiments with 2 or 3 livers per genotype. Fetal liver chimeras were generated by transplanting Klf7^+/+, Klf7^−/+, or Klf7^−/− fetal liver (Ly5.2) cells with an equal number of WT competitor fetal liver (Ly5.1) cells into irradiated congenic (Ly5.1/5.2) mice. (C) The percentage of donor leukocytes at the indicated times. (D) The contribution of donor cells to B cells (B220⁺), T cells (CD3e⁺), neutrophils (Gr-1 high), and monocytes (Gr-1⁺ CD115⁺) assessed 12 weeks after transplantation is shown for 1 representative experiment. Bone marrow was harvested from primary recipients at 12 weeks and serially transplanted. Shown is donor contribution in secondary (E) or tertiary (F) recipients. Data represent 2 or 3 independent experiments for each genotype, with 4 or 5 mice per genotype per experiment.