KLF7 overexpression inhibits in vitro myeloid colony formation. WT bone marrow cells from 5-fluorouracil–treated donors were transduced with MSCV-IRES-GFP expressing KLF7 or vector alone. (A) Transduced (GFP⁺) cells were isolated 72 hours after transduction, and the expression of KLF7 (relative to β-actin) was measured by real-time RT-PCR. Data represent 2 independent experiments. *P = .033. Unsorted bone marrow cells exposed to control or KLF7 retrovirus were plated in methylcellulose and cultured for 7 days. (B) Representative photomicrograph showing the GFP⁺ colonies (top panel) and total colonies (bottom panel). Original magnification ×10. (C) The percentage of GFP⁺ colonies relative to the input percentage of GFP⁺ cells. *P = .026. Data represent the average ± SEM of 2 independent experiments, each plated in duplicate. (D) GFP⁺ sorted cells from lentivirally transduced c-Kit⁺ cells were plated in complete methylcellulose, and colonies were scored by morphology after 7 days of growth.