KLF7 overexpression suppresses hematopoietic stem and progenitors. Bone marrow stem and progenitor populations were analyzed by flow cytometry 6 weeks after transplantation with control or KLF7 lentivirally transduced cells. (A) Gating strategy used to identify HSCs (KLS CD34−), common myeloid progenitors (CMP, Lin− c-Kit+ Sca-1− CD16/32lo CD34+), granulocyte-monocyte progenitors (GMPs, Lin− C-Kit+ Sca-1− CD16/32hi CD34+), and megakaryocytic-erythroid progenitors (MEP, Lin− c-Kit+ Sca-1− CD16/32lo CD34−; top panel) and common lymphoid progenitors (CLP, Lin− CD27+ IL7R+ Flk-2+ Ly6D−; bottom panel). Cells are first gated on live, donor, and lineage-negative populations. (B) Representative histograms showing GFP expression in the indicated progenitor cell population. (C) A summary of the percentages of donor cells that are GFP+ for the indicated progenitor population is provided. Input refers the percentage of bone marrow cells that were GFP+ at the time of transplantation. Data represent the mean ± SEM of 2 independent experiments. ***P < .001.
