KLF7 overexpression supports early thymocyte development. Blood, spleen, and thymic T-cell populations were analyzed by flow cytometry 6 weeks after transplantation with control or KLF7 lentivirally transduced cells. (A) Representative flow plot for the thymus, with each of the 4 major thymic populations (CD4⁺ CD8⁺ [DP], CD4⁻ CD8⁻ [DN], CD4⁺ CD8⁻ [CD4 SP], and CD4⁻ CD8⁺ [CD8 SP]) subsequently gated for GFP. (B) CD4 SP and CD8 SP T-cell subsets in the blood and spleen and DN, DP, CD4 SP, and CD8 SP subsets in the thymus as gated in the representative flow plot (A) were analyzed, and the percentage of GFP⁺ for each population from each tissue is shown. Input refers to the percentage GFP⁺ cells at the time of transplantation. *P < .05 compared with input values. (C) GFP⁺ thymic populations as sorted in panel A were analyzed for annexin V and DAPI staining. The results are summarized in panel D, showing the percentage of the GFP⁺ fraction of the indicated thymic population that were annexin V⁺. Data represent 2 independent experiments with 4 mice per condition. *P < .05.