Type 1 IFN gene targets in CD8 T cells prepared on day 8 of LCMV infection. CD8 T cells were purified from uninfected (D0) or D8 LCMV-infected WT mice and treated with either control or IFNα for 90 minutes. The RNA was extracted and analyzed in comparison with D0 samples as in Figure 1A and B. Real-time PCR analysis of STAT1, Mx2, c-myc, and IFNγ mRNA was performed. Black bars show results with control-treated and gray bars show results with IFNα-treated CD8 T-cell samples (C). (D) CD8 T cells were treated with formaldehyde to crosslink DNA-bound transcription factors to DNA. STAT1 or STAT4 promoter binding was evaluated by chromatin immunoprecipitation and qPCR with custom primers (see “Chromatin immunoprecipitation” and “Real-time PCR”). Where shown, bars represent SEMs. Results are based on accumulated data from 3 or more replicates.