Figure 2
Figure 2. Visualization of intracellular NO production in RBCs. (A) Laser scanning micrographs of RBCs loaded with DAF-FM before (top) or after pretreatment with 3mM L-NAME (inset) and further incubation with 100μM Sper/NO (bottom). Please refer to “Methods” for details. (B) Fluorescence distribution plot of untreated cells (front lane), cells loaded with DAF-FM only (second lane), or further treated with 1 to 500μM SNOC (other lanes). Representative data from 6 experiments (C) Decrease in intracellular RBC fluorescence (ΔMFI) as assessed by flow cytometry after treatment with the NOS inhibitor L-NAME. (D) Intracellular RBC fluorescence (ΔMFI) after treatment with the NO scavenger Fe[DETC]2. ΔMFI = median fluorescence intensity − background fluorescence.

Visualization of intracellular NO production in RBCs. (A) Laser scanning micrographs of RBCs loaded with DAF-FM before (top) or after pretreatment with 3mM L-NAME (inset) and further incubation with 100μM Sper/NO (bottom). Please refer to “Methods” for details. (B) Fluorescence distribution plot of untreated cells (front lane), cells loaded with DAF-FM only (second lane), or further treated with 1 to 500μM SNOC (other lanes). Representative data from 6 experiments (C) Decrease in intracellular RBC fluorescence (ΔMFI) as assessed by flow cytometry after treatment with the NOS inhibitor L-NAME. (D) Intracellular RBC fluorescence (ΔMFI) after treatment with the NO scavenger Fe[DETC]2. ΔMFI = median fluorescence intensity − background fluorescence.

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