ERG regulates expression of AML1-ETO target genes. (A) Overview of the RUNX1, FLI1, and ERG upstream regulatory regions in SKNO-1 cells. Orange represents the RUNX1 data; red, the AE data; yellow, the ERG data; and pink, the FLI1 ChIP-seq data. (B) Schematic representation of a suggested positive feed forward loop between RUNX1 and FLI1/ERG and the potential interference of AML1-ETO. (C) Western analysis of ERG expression in wt and ERG silenced (+ dox) SKNO-1 cells. (D) Expression level (as assessed by RPKM values) of ERG, RUNX1, FLI1, and AML1-ETO in wt SKNO-1 cells and SKNO-1 cells that express different ERG silencing constructs. (E) Median RPKM values of ERG target genes bound (black) or not bound (gray) by AML1-ETO in wild-type SKNO-1 cells compared with 2 ERG silenced (+ dox) SKNO-1 cell lines. (F) Heatmap showing expression changes in genes bound by AML1-ETO/ERG at the promoter or within intragenic regions.