American Society of Hematology

Figure 6

$Figure 6. αTAT and mTAT ELISA analyses of PPP reconstituted with washed RBCs. WB was drawn via phlebotomy into 0.1 mg/mL of CTI, centrifuged at 150g, and subsequently washed as described in “Methods.” The same subject's blood was redrawn into 0.1 mg/mL of CTI and a fraction was centrifuged to obtain PPP. Three fractions: WB (○), PPP (▴), and PPP + washed RBCs (■), were then subjected to 5pM relipidated rTf. Samples were quenched at various intervals with EDTA and Phe-Pro-Arg chloromethylketone and analyzed via αTAT ELISA (A) or mTAT ELISA (B). Data are shown as the means ± SEM (n = 3).$

αTAT and mTAT ELISA analyses of PPP reconstituted with washed RBCs. WB was drawn via phlebotomy into 0.1 mg/mL of CTI, centrifuged at 150g, and subsequently washed as described in “Methods.” The same subject's blood was redrawn into 0.1 mg/mL of CTI and a fraction was centrifuged to obtain PPP. Three fractions: WB (○), PPP (▴), and PPP + washed RBCs (■), were then subjected to 5pM relipidated rTf. Samples were quenched at various intervals with EDTA and Phe-Pro-Arg chloromethylketone and analyzed via αTAT ELISA (A) or mTAT ELISA (B). Data are shown as the means ± SEM (n = 3).

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